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caco 2 cells monolayer  (ATCC)


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    ATCC caco 2 cells monolayer
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Caco 2 Cells Monolayer, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 16805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells"

    Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1671729

    Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Figure Legend Snippet: Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

    Techniques Used: Permeability, Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY

    Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.
    Figure Legend Snippet: Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY



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    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
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    Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

    Journal: Frontiers in Immunology

    Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

    doi: 10.3389/fimmu.2025.1671729

    Figure Lengend Snippet: Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

    Article Snippet: For all experiments, we used a well validated model of gut barrier based on Caco-2 cells monolayer (American Type Culture Collection, Middlesex, UK; accession number: HTB-37) ( , ).

    Techniques: Permeability, Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY

    Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

    Journal: Frontiers in Immunology

    Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

    doi: 10.3389/fimmu.2025.1671729

    Figure Lengend Snippet: Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

    Article Snippet: For all experiments, we used a well validated model of gut barrier based on Caco-2 cells monolayer (American Type Culture Collection, Middlesex, UK; accession number: HTB-37) ( , ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY

    Representative micrographs (A) uninfected Vero cells; (B) Cooper-1 CPE; (C) field sample (no CPE).

    Journal: Frontiers in Veterinary Science

    Article Title: Serological and molecular surveillance of infectious bovine rhinotracheitis in Kazakhstan

    doi: 10.3389/fvets.2025.1734128

    Figure Lengend Snippet: Representative micrographs (A) uninfected Vero cells; (B) Cooper-1 CPE; (C) field sample (no CPE).

    Article Snippet: Virus isolation attempts were conducted on all rRT-PCR–positive samples from 2024–2025 using Vero cell monolayers (ATCC CCL-81).

    Techniques: